Journal: PLoS ONE

Article Title: In vitro Study of a Novel Stent Coating Using Modified CD39 Messenger RNA to Potentially Reduce Stent Angioplasty-Associated Complications

doi: 10.1371/journal.pone.0138375

Figure Lengend Snippet: Flow cytometric analyses of transfected HEK293 cells (A) human ECs (B) and immortalized porcine endothelial cells (PAE; C) with different CD39 mRNA concentrations. For control untreated and Lipofectamin-treated cells were used. The cells were labeled with a CD39-FITC antibody 24 h after transfection. Each experiment was performed at least 3 times. Data are given as means and SEM and were compared using one-way ANOVA (A & B) or RM-ANOVA (C) with Bonferroni´s multiple comparison test. ***p < 0.001. Representative fluorescence microscopic images of CD39 mRNA transfected (C, 2 μg CD39 mRNA) and untransfected (D) HEK293 cells.

Article Snippet: For transfection of the PAE cells (SibTech, Brookefield, USA) the double amount of cells were seeded and hence twice the amount of the following transfection components were used.

Techniques: Transfection, Control, Labeling, Comparison, Fluorescence